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General information Notification Number B/SE/23/3093 Member State to which the notification was sent Sweden Date of acknowledgement from the Member State Competent Authority 24/02/2023 Title of the Project Potato with altered resistance to pathogens or yield Proposed period of release: 15/05/2023 to 15/10/2027 Name of the Institute(s) or Company(ies) SLU, Plant protection Biology, Horticum, Växtskyddsvägen 1 Lomma Sweden 23456
Is the same GMPt been notified elsewhere by the same notifier? No Has the same GMPt been notified elsewhere by the same notifier? No Genetically modified plant Complete name of the recipient or parental plant(s):

Common Name	Family Name	Genus	Species	Subspecies	Cultivar/breeding line
potato	solanaceae	solanum	solanum tuberosum	tuberosum

Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications: A) DNA-free mutation of DMR6 gene. No selection.B) Overexpression of Psi1 gene, with neomycin phosphotransferase II (nptII) coding for kanamycin resistance as a marker gene.C) Overexpression of mCherry - control to Psi1. Neomycin phosphotransferase II (nptII) encoding kanamycin resistance as a marker gene.D) Overexpression of violaxanthin de-epoxidase (VDE), PSII subunit S (PsbS) and zeaxanthin epoxidase (ZEP). Neomycin phosphotransferase II (nptII) encoding kanamycin resistance as a marker gene Genetic modification Type of genetic modification: Insertion;Other; Other

DNA-free mutation (A) In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted: B) PiS1ox T-DNA: Left border (LB) from Agrobacterium tumefaciens, nopaline synthase polyadenylation sequence (nosT) from Agrobacterium tumefaciens, neomycin fosfotransferase II (Kan) which can be isolated from different bacteria, nopaline synthase promotor (Pnos) from Agrobacterium tumefaciens, 35S promotor (P35S) from cauliflower mosaic virus (CaMV), recombination sequence from GATEWAY vector (Invitrogen), PCR amplified PiS1-gene from Nicotiana benthamiana in frame with mCherry, recombination sequence from GATEWAY vektor (Invitrogen), polyadenylation sequence from cauliflower mosaic virus (CaMV) 35S transcript (T35S), right border (RB) from pTiT37 from Agrobacterium tumefaciens. C) EvCherry T-DNA: Left border (LB) from Agrobacterium tumefaciens, nopaline synthase polyadenylation sequence (nosT) from Agrobacterium tumefaciens, neomycin fosfotransferase II (Kan) which can be isolated from different bacteria, nopaline synthase promotor (Pnos) from Agrobacterium tumefaciens, 35S promotor (P35S) from cauliflower mosaic virus (CaMV), recombination sequence from GATEWAY vector (Invitrogen), mCherry red fluorescent protein gene, recombination sequence from GATEWAY vector (Invitrogen), polyadenylation sequence from cauliflower mosaic virus (CaMV) 35S transcript (T35S), right border (RB) from pTiT37 from Agrobacterium tumefaciens. D) pVPZ T-DNA Left border (LB) from Agrobacterium tumefaciens, A. thaliana RuBisCO small subunit 1A (AT1G67090) promoter (Rbcs1A-P), A. thaliana Zeaxanthin epoxidase (AT5G67030.1) (AtZEP), A. thaliana Heat shock protein 18.1 terminator (HSP1.8-T), A. thaliana Glyceraldehyde 3-phosphate dehydrogenase A subunit 1 (AT3G26650) promoter (GAPA-P), A. thaliana Photosystem II subunit S (AT1G44575.1) (AtPsbS), A. thaliana Heat shock protein 18.1 terminator (HSP1.8-T), A. thaliana Fructose-bisphosphate aldolase 2 (AT4G38970) promoter (FBA2-P), A. thaliana Violaxanthin de-epoxidase (At1G08550.1) (AtVDE), A. thaliana Heat shock protein 18.1 terminator (HSP1.8-T), Agrobacterium tumefaciens nopaline synthase promotor (Pnos), neomycin fosfotransferase II (Kan) which can be isolated from different bacteria, Agrobacterium tumefaciens octopine synthase terminator (ocs_T). Brief description of the method used for the genetic modification: A) For transfection, a DNA-free system has been used. A nuclease, Cas9, catalyzes double-strand breaks (DSB) in the genome that are repaired by the potato's own repair system, "non homologous end joining". At times, small genetic changes produced by NHEJ repair consisting of 'indels' occur. Cas9 is guided to the gene of interest via an RNA guide B, C, D) For transformation of potato a binary vector system where sequences to be transferred can be found inside the border sequences that form a transfer DNA (T-DNA) was used. The DNA mobilization features are available in a modified Ti plasmid that is not transferred to the plant. For transformation of T-DNA to the potato, Agrobacterium tumefaciens containing the vector has been used. Cut potato leaf tissue was transformed and transgenic shoots were selected on antibiotics. After transformation, the Agrobacterium killed with Cefotaxime. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination: no Experimental Release Purpose of the release: A, B, C) Potatoes are grown on 1% of agricultural land, but as much as 30% of all fungicides used in Sweden are sprayed on potatoes. Cultivation of resistant potatoes would provide great environmental benefits as the amount of fungicide used could be reduced. D) Due to future global food demand the current crop yields per hectare of land are inadequate and therefore crop productivity needs to be improved. Data suggest that by using plants where the is limitations in the photosynthetic process have been is a viable strategy for increasing crop yield. The purpose is to evaluate agricultural value under field conditions including resistance characteristics. Study the stability of the modified traits, identify any morphological deviations, produce field-grown material for laboratory trials, and produce seed for next year's field trials. The trial is for research purposes only Geographical location of the site: Sweden (Lomma, Kristianstad, Kävlinge, Umeå, Båstad, Öland, Gotland) Size of the site (m2): <10000M2 per site. Maximum 7x10000m2 Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release: n/a Environmental Impact and Risk Management Summary of the potential environmental impact from the release of the GMPts: Note especially if the introduced traits could directly or indirectly confer an increased selective advantage in natural environments; also explain any signifant expected environmental benefitsPotato tubers are frost-sensitive and thus depend on temperature to be able to overwinter in the field. Overwintering of tubers can take place in southern Sweden after a mild winter. Seeds can survive regardless of temperature. Very rarely do waste plants of potatoes occur in the cultivated landscape as the seeds and any tubers are destroyed by the next year's tillage, the use of herbicides and competition from the following crop. Potatoes only appear in the cultivation landscape. There have been no reports of wild potato plants. Potatoes also have no compatible relatives in Europe and can therefore only interbreed with other cultivated potatoes. The reported dispersal distance for potato pollen is very short and the suggested distance of 20 m to other cultivated potatoes exceeds the dispersal distance and is therefore considered sufficient to prevent inadvertent crossing. As potato flowers lack nectar, pollen dissemination by honeybees is unlikely and they tend not to forage in potato crops. Flower buds will be removed. Brief description of any measures taken for the management of risks: The experiment will be carried out by the local Rural Economy and Agricultural Society (here abbreviated HS) with the help of members of the Resistance Biology group at VSB, SLU. Both have extensive experience in GM field trials.The experiment will be inspected by HS at least once a week. Responsible scientists will inspect the field during cultivation period. Monitoring of fields after harvest will be carried out by HS according to a mutual written agreement. In order to check for any surviving tubers after harvest, other potato will not be grown in the fields until at least one growing season without surviving potatoes is observed.In order to minimize the spread of pollen to other cultivated potatoes (according to the literature, the distance for the spread of potato pollen is a maximum of ten meters), a protective distance of 20 m will be maintained. All flower buds will be removed. Cleaning of machines, tools and transport vehicles will take place after contact with modified lines. Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release: Not applicable Final report - European Commission administrative Information Consent given by the Member State Competent Authority: Yes
04/28/2023