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General information Notification Number B/Be/23/V4 Member State to which the notification was sent Belgium Date of acknowledgement from the Member State Competent Authority 18/12/2023 Title of the Project R&D Field trial to evaluate the phenotype and yield of maize lines gene edited for optimised plant architecture. Proposed period of release: 15/04/2024 to 10/12/2024 Name of the Institute(s) or Company(ies) INARI Agriculture N.V., Belgium
Is the same GMPt been notified elsewhere by the same notifier? No Has the same GMPt been notified elsewhere by the same notifier? No Genetically modified plant Complete name of the recipient or parental plant(s):
Common Name Family Name Genus Species Subspecies Cultivar/breeding line
maize poaceae zea zea mays mays
Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications: The edited maize lines have a shorter stature and larger biomass due to an edit in a transcription factor gene and a transcriptional coactivator gene resulting in an altered expression. Both edits impact together internode elongation and, hence, plant height and biomass quantity. Genetic modification Type of genetic modification: Other; Brief description of the method used for the genetic modification: The plants have been modified using CRISPR-Cas. A maize inbred variety was transformed with Cas. Via biolistic transformation of immature maize kernels the native genetic element, specific designed guide RNAs, and a plasmid encoding a visual marker and a selectable marker cassette were introduced in the maize line. Plants with the desired edits were selected and all other elements were segregated out in the next generation. The plants no longer contain any foreign genetic material. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination: Not applicable Experimental Release Purpose of the release: Analysis of the phenotype and yield potential of the edited maize plants under field conditions. Geographical location of the site: ILVO research institute in the municipality of Wetteren, East-Flanders. Size of the site (m2): 1710 m2 including border rows of conventional maize Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release: One of the gene edited lines was tested in the field during Summer 2023. The expected phenotype was observed and no data indicate any potential impacts on the environment and/or human health. Environmental Impact and Risk Management Summary of the potential environmental impact from the release of the GMPts: Note especially if the introduced traits could directly or indirectly confer an increased selective advantage in natural environments; also explain any signifant expected environmental benefitsThe potential environmental impact of the edited maize lines is expected to be negligible. The modification has no effect on survivability or persistence. There is no effect on the mode of reproduction. The edited maize can cross with other maize. Maize has no sexually compatible relatives in Belgium. The modification does not target any organism or effect herbivores or other non-target organisms. No health impacts are expected on the people handling the edited maize plants, nor effects on biogeochemical processes. Brief description of any measures taken for the management of risks: Special care will be taken for any seed material be transported to and from the trial site.Border rows of conventional maize will be planted around the actual field trial as pollen catch plants. The trial will be located at least 200 m away from any other maize. Furthermore, a 1.80 m high wire fence will surround the field trial to prevent accidental intruding and accidental removal or spread of modified maize material.At the end of the trial, all plants, including the conventional border rows, will be harvested and inactivated. Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release: Not applicable Final report - European Commission administrative Information Consent given by the Member State Competent Authority: Not known
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