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General information Notification Number B/SE/23/4198 Member State to which the notification was sent Sweden Date of acknowledgement from the Member State Competent Authority 16/03/2023 Title of the Project Arabidopsis - photosynthesis and hormone biology Proposed period of release: 01/06/2023 to 30/09/2027 Name of the Institute(s) or Company(ies) Umeå University, Dept. Of chemistry
Is the same GMPt been notified elsewhere by the same notifier? No Has the same GMPt been notified elsewhere by the same notifier? No Genetically modified plant Complete name of the recipient or parental plant(s):

Common Name	Family Name	Genus	Species	Subspecies	Cultivar/breeding line
cress	brassicaceae	arabidopsis	arabidopsis thaliana		Columbia (Col-0)

Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications: In one case,changed regulation of the photosynthetic light harvesting, specifically the so-called "non-photochemical quenching" (NPQ), a kind of safety valve against excess light. The PsbS protein is the key protein for the process, and here the PsbS protein is over-expressed. In the other cases are the lines part of a very ambitious project to understand the functions of the ABP1-protein. ABP1 was for many years believe to be a receptor for the plant hormone aunix, but a few years ago data were published that this was not the case. However, very recent data show that it may be an auxin-receptor after al. ABP1 interacts with several other proteins, TMK1, ABF2 and TIR1. The lines covered by this applications has mutations in one (or more) of these key genes (in some cases EMS, in some CRISPR, in some T-DNA KO), all of these will be impaird in the auxin response, Most of the genetic transformations are made to restore the wild-type functions of the mutants, though reintruduction of a gene copy with wild-type function (in a few casese with variants of the gene product). These plants may or may not regain wildtype properties (this is what we will study). In addition, resistance genes for selection during transformation have been introduced   Genetic modification Type of genetic modification: Insertion;Deletion; In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted: A} PsbS gene under own promoter to increase non-photochemical quenching (NPQ), gentamycin resistance B) No insertion (CRISPR-mutations in ABP1) C) One line from B complemented with wildtype version of the gene, fused to GFP. Kanamycin-resistance gene for selection. D) Kanamycin resistance gene for selection, that has knocked out genes in auxin signalling, TMK1 or AFB2. E) One of the tmk-lines from D complemented with wildtype version of the gene (fused to FLAG) and hygromycin resistance gene for selection F) an EMS-mutation in the TIR1 gene (not covered by GMO regulation) complemented with either the wildtype version of the gene, or wildtype version with a point mutation. BASTA resistance gene for selection. These lines also contain the AFB2 KO-mutation from D) above In case of deletion of genetic material, give information on the function of the deleted sequences: in B) 5 bp deletion knocking out the ABP1 gene Brief description of the method used for the genetic modification: Agrobacterium-mediated transformation If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination: no Experimental Release Purpose of the release: Basic research on photosynthesis and hormone biology Geographical location of the site: Umeå university Size of the site (m2): 10 Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release: no Environmental Impact and Risk Management Summary of the potential environmental impact from the release of the GMPts: Note especially if the introduced traits could directly or indirectly confer an increased selective advantage in natural environments; also explain any signifant expected environmental benefitsNo potential impact. The line overexpressing PsbS has been studied extensively over 20 years, the take-home message is that such plants by large perform like wild-type plants, to worse or no better. Concerning all the lines with impaired hormome perception; the puropse of the whole experiment is to see if plants with a mutation in a key genes for auxin metabolism still can thrive in the field, although they would be supposed to not be so fit. If they would turn out to be less fit, can the wild-type function be restored by the introduction of a wild-type gene (or a variant thereof)? It is almost imporssiblle for us to imagine how this could lead to an selective advantage, only disadvantages. Brief description of any measures taken for the management of risks: Daily care, collection of all plant material, soil etc as we have done in previous experiments (see Frenkel et al. 2008, attached). Sowing indoors, growth in pallets (often covered), plants are followered one by one, samples are taken and at the end of the exeriment all material (inclusing soil) is treated as transgenic Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release: no Final report - European Commission administrative Information Consent given by the Member State Competent Authority: Yes
06/19/2023