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General information Notification Number B/SE/22/23780 Member State to which the notification was sent Sweden Date of acknowledgement from the Member State Competent Authority 20/12/2022 Title of the Project Potato with decrease in antinutritionel compounds. Potato with tailored starch quality. Potato with decreased susceptibility to pathogens. Proposed period of release: 10/04/2023 to 15/11/2027 Name of the Institute(s) or Company(ies) Sveriges Lantbruksuniversitet, Umeå Plant Science Centre, Skoglig genetik och växtfysiolog
Is the same GMPt been notified elsewhere by the same notifier? No Has the same GMPt been notified elsewhere by the same notifier? No Genetically modified plant Complete name of the recipient or parental plant(s):

Common Name	Family Name	Genus	Species	Subspecies	Cultivar/breeding line
potato	solanaceae	solanum	solanum tuberosum	tuberosum	Kuras, Saprodi, Allstar, Desiree, Fyone, Ydun

Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications: The genotypes produced has one of below three traits introduced.Potatoes with altered starch quality:Starch consists of two components, amylose and amylopectin. Both molecules are made up of glucose molecules but differ in that amylopectin is a large and branched molecule while amylose is a mostly linear and long molecule. The linerar chains of the starch molecules are synthesized by starch synthases (SS). A granule-bound starch synthase (GBSS) builds the chains that become amylose while soluble starch synthases (SSS) build chains found in amylopectin. The branches of the starch molecules are synthesized by branching enzyme (SBE). SSS exists in different forms (I-IV), and its functions differ marginally by building amylopectin chains of different lengths. In the potato lines covered by the application, GBSS, SSS and SBE have been mutated to change the chain length and degree of branching of amylose and amylopectin. Genetic modification Type of genetic modification: Other; Other

Mutagenesis through small indels Brief description of the method used for the genetic modification: The potatoes have been developed through DNA-free mutagenesis. A nuclease, Cas9, catalyzes double-stranded breaks (DSB) of the genome. THe DSBs are repaired by the potato's own repair system, "non homologus end joining" (NHEJ). On some occasions, small genetic changes arising from the NHEJ repair occur of small "indels". Cas9 has been guided to the gene of interest via an RNA guide. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination: NO Experimental Release Purpose of the release: The purpose of the release of gene edited potatoes is to;Study any morphological changes that may arise via somatic variation as a result of in-vitro cultivation.Study the yield of the lines and the starch content of the mature tubers as this is not possible in a greenhouse.Study the lines' susceptibility to biotic and abiotic stress.Extract starch from the tubers for further use in various application studies. Long-term goals are variety testing and marketing. Geographical location of the site: Skåne and Blekinge. Additionally, municipalities of Torsås, Kalmar, Mörbylånga, Borgholm, Mönsterås, Skara, Lidköping, Mariestad, Vara, Götene and Uppsala. Size of the site (m2): Maximum of 5000 ha Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release: Potatoes with altered starch quality produced with gene editing have been grown in field trials since 2017. No increased risk to health or the environment has been identified during the field trials. Another potato (Eliane) lacking amylose produced by mutagenesis is grown commercially and its starch is marketed by AVEBE. Eliane is exempt from GM regulation. Lines with reduced degree of branching and reduced susceptibility produced with traditional GMO techniques have been grown in field trials before. No increased risk to health or the environment has been identified during the field trials. Environmental Impact and Risk Management Summary of the potential environmental impact from the release of the GMPts: Note especially if the introduced traits could directly or indirectly confer an increased selective advantage in natural environments; also explain any signifant expected environmental benefitsThe modified tubers are not predicted to provide any increased survivability.Previous studies of potatoes with a lack of amylose, reduced or increased chain length and reduced degree of branching of the starch have shown that the modification has no effect on tubers and seeds.Potato tubers with a reduced content of glycoalkaloids can be speculated to give an increased sensitivity to biotic stress. No increased survivability is expected.Potato tubers with reduced susceptibility to pathogens are expected to provide a reduced impact of biotic stress during the early growing season. The lines do not have full resistance but a reduced and delayed infection. As potatoes in conventional crops are sprayed against pathogens, no difference in survival is expected compared to potatoes from these crops where pathogen infections are prevented during the season. Brief description of any measures taken for the management of risks: In order to minimize the spread of pollen to other cultivated potatoes (according to the literature, the distance for the spread of potato pollen is a maximum of ten meters) (OECD, 1997), a protective distance of 20 m will be applied. Honeybees (Apis mellifera) are not pollinators of potatoes, as potato flowers do not contain nectar (OECD, 1997). Potato pollen is therefore assumed not to spread via pollinators.Cleaning of machines, tools and transport vehicles will take place after planting and harvesting.No potatoes will be grown on the trial area the following year. The following cultivations will take place in such a way that no part of the potato can enter the food or feed chain.The trial will be inspected by responsible growers at least once every two weeks. Assessment and actions will be made regarding protection against disease attacks and wild animal attacks.The potatoes are not expected to pose an environmental risk. If an emergency nevertheless should arise, the lines will be eliminated mechanically or with herbicides. Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release: Not applicable Final report - European Commission administrative Information Consent given by the Member State Competent Authority: Yes
03/27/2023