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General information Notification Number B/ES/23/13 Member State to which the notification was sent Date of acknowledgement from the Member State Competent Authority 01/01/1970 Title of the Project Using transgenic plants plum as rootstocks of commercial varieties of peach and apricot Proposed period of release: 01/01/1970 to 01/01/1970 Name of the Institute(s) or Company(ies) CEBAS-CSIC, Campus Universitario de Espinardo, Edificio nº 25 30100 Murcia
Is the same GMPt been notified elsewhere by the same notifier? No Has the same GMPt been notified elsewhere by the same notifier? No Genetically modified plant Complete name of the recipient or parental plant(s):

Common Name	Family Name	Genus	Species	Subspecies	Cultivar/breeding line
apricot	rosaceae	prunus	prunus armeniaca		Seeds from Stanley

Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications: The strain EHA105, carrying the binary vector h-UTR/P1 was used for inoculation of hypocotyl slices as described in Petri et al., (2008). The cDNA codes for a 759bp fragment of the virus genome close to 5’ region. The resulting construction also harboured in the T-DNA the neomycin phosphotransferase (nptII) gene for aminoglycoside selection by adding kanamycin to the culture media (Figure 1).The plasmid introduced in Agrobacterium was proportionated by Dr. Vincenza Ilardi and is detailed described in Di Nicola-Negri et al. (Transgenic Res 14: 989-994, 2005).  Genetic modification Type of genetic modification: Insertion; In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted: The construction has a transgene coding for a hairpin with an intron (ihpRNA) covering part of the non-translated region of 5’ end and a portion of the P1 gene from the strain ISPaVe44 of an Italian PPV-M (Figure 1), that triggers a post-transcriptional silencing specific for that virus genome region. The T-DNA used contains between right and left borders a nptII gene conferring aminoglycoside antibiotics resistance under the control of a NOS promotor and terminator.   Brief description of the method used for the genetic modification: We used the transformation protocol described by Petri et al. (Mol Breed 2008; 22: 581-91) for European plum hypocotyls mediated by Agrobacterium tumefaciens. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination: Not applicable Experimental Release Purpose of the release: We aim to investigate the behavior of the GMHP (Genetically Modified Higher Plants) as a rootstock for different commercial varieties of Prunus under the regular cropping and environmental conditions of Murcia. These GMHP will be grafted with ‘Canino’ apricot, ‘Levante 45’ peach and ‘Red Beauty’ Japanese plum onto lines St5’-1, St5’-9 and Stanley seedlings as a control.Since the GMHPs will be released in a well-defined experimental cropping plot, no invasion of natural habitats and no gene transfer are expected, since GMHPs will not develop reproductive organs, and that will be used only as a rootstock.The interaction with other living organisms is minimal, and common control measures will be applied in order to prevent infection by fungi, bacteria and plant viruses. Furthermore, the interaction of both humans and natural predators with these crops is basically due to the intake of fruits, which will be not produced in any case for the GMHPs. Finally, interaction between the GMHPs and soil microorganisms has not been described. Geographical location of the site: Blanca, Murcia Size of the site (m2): Aproximately 400 m2 Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release: There have been no previous releases. Environmental Impact and Risk Management Summary of the potential environmental impact from the release of the GMPts: Note especially if the introduced traits could directly or indirectly confer an increased selective advantage in natural environments; also explain any signifant expected environmental benefits Brief description of any measures taken for the management of risks: There will be no risk of dispersion because the GMHPs will be used as rootstock and reproductive organs will be developed. The release site will be visited regularly by staff of the Experimental Filed (2-3 times per week) and by members of the research team (1 time per month). With regard to pruning, this will be performed by trained personnel of the Experimental Field of CEBAS-CSIC, which, together with the research team, is responsible for overseeing the development of GMHPs in order to avoid the formation of reproductive organs. The pruning material will be incinerated in a controlled manner at the premises of the Experimental Filed of CEBAS-CSIC, Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release: Not applicable Final report - European Commission administrative Information Consent given by the Member State Competent Authority: Not known